The main research focus of the project concentrates on structural- and signal transduction pathway analyses of the membrane bound CD83 molecule. Specific binding domains/-partners have been identified using a yeats two hybrid screen. Site directed mutagenesis-, transfection-, immune-precipitation-, and co-immunofluorescence-studies have been used to further characterize the protein-protein interaction, the N-linked glycosylation, and the activation of mCD83 on a molecular level. To identify possible binding motifs in silico, a bioinformatic modeling study has been performed. The elucidation of the mCD83 signaling pathway in mature human DC will open new and specific therapeutic targets.